Virtual Physiology

the unique truly simulation software

physiology and pharmacology experiments
in virtual laboratories
almost like in the real world
- Perfect for online teaching and remote learning -

Student Licenses - in Times of the Corona Virus

As our users know, we are sending, whenever requested, additional time-limited license files for students to llicet them install the programs on their own computers for experimentation at home.
This may become particularly helpful when the corona virus does not allow coming together in regular practical courses.
Please note that also each institute which wants to check the potential value of our virtual laboratories can get such time-limited licenses (e.g. for 2 months) that can be forwarded to their students. Just send a short note to braun (at) uni-marburg.de, including the name and address of your institution.

The Virtual Laboratories:

The Virtual Physiology programs are going far beyond conventional teaching tools, offering realistically appearing, fully equipped laboratories on the computer screen for free experimentation.

All stimulation and recording devices are freely adjustable. Mathematical algorithms guarantee for the appropriate reactions of the preparations in all situations, also considering their physiological diversity.

SimHeart and SimVessel feature physiological and pharmacological experi-ments with the isolated, perfused heart in the Langendorff-setup and with isolated smooth muscle strips of blood vessels (aorta) and the stomach (antrum) to examine the muscle contractions after application of physiolo-gical transmitters (Acetylcholine), hormones (Adrenaline) and diverse drugs like the competitive receptor antagonists (Atropine, Phentolamine, Propranolol) and non-competitive modulators of Ca2+-currents and Ca2+-concentrations (Verapamil, g-Strophantin). An additional “Drug Laboratory” can be used practicing the correct preparation of the requested dilutions.

SimMuscle and SimNerv offer highly realistic experimental set-ups on the computer screen to record contractions of the frog’s gastrocnemic muscle (e.g. single twitches vs. tetanic contractions, muscle fatigue, curves of isometric and isotonic maxima) or compound action potentials from the frog’s sciatic nerve (dependence on electrode positions, refractory period, anode break potentials, etc.), respectively.

SimNeuron additionally allows performing widely used voltage- and current-clamp experiments in an easy to overlook lab design for recordings of current-voltage curves (reversal potentials etc.) or the threshold of action potential generation and its dependencies on stimulus strength and duration – also under application of TTX or TEA.

Didactic Aspects:

Although the virtual laboratories have originally been considered as minor substitutes of experiments with animal preparations, it turned out that the virtual labs can have major didactic advantages:

- The students are doing the experiments without negative emotions from the killing of an animal and without the fear that they need to kill one more animal if they are making a mistake that destroys the preparation.

- Only in the virtual laboratories the students feel free to explore the reaction of the preparation on their own, including unconventional stimulation protocols.

- The students have the possibility studying the reactions of the virtual preparations without any time pressure - also on their computers at home.

- Technically more challenging experiments like voltage- and patch-clamp recordings, that are unfeasible to be physically carried out in students’ introductory courses, can be realized in silico with a user-friendly interface.

Programs

running on all Windows platforms,
from Win XP to Win 10, 32 bit as well as 64 bit versions
SimHeart and SimVessel also include .swf files to run on MacOS
(see Technical Specifications)

problems with Win 10?
a) concerning hidden task bars of the Virtual Physiology programs in the full screen mode
b) concerning strange sound effects (only in very recent Win 10 versions appearing).

SimHeart©

SimHeart offers a virtual laboratory for recordings of heart contractions in the Langendorff set-up in response on the most relevant transmitters and drugs, including a drug laboratory for the adjustment of the appropriate solutions.

Featured experiments:

Effects of Adrenaline and Acetylcholine on frequency and amplitude of heart contractions (inotroph and chronotroph effects)
Effects of competitive receptor blockers Propranolol and Atropine
Aadrenaline dose-response curve and its shift by the ß-blocker Propranolol (competitive inhibition)
Comparison with non-competitive inhibition by the Ca²⁺-channel blocker Verapamil
Strengthening of the contractions by the heart-glycoside g-Strophantine
Induction and treatment of arrhythmias and heart blocks in systole and diastole

for details see Tutorial and Protocol form

download a fully functioning DEMO Version

SimVessel©

SimVessel offers a virtual laboratory for the examination of smooth muscle contractions of vessels and the intestine.
The experiments can be done with muscle stripes, placed in an organ bath to which physiologically relevant signal substances and widely used drugs can be added. Preparing the appropriate dilutions can be trained, as in SimHeart, in a drug laboratory.

Featured experiments:

Comparison of phasic and tonic smooth muscle contractions.
Illustrating the effects of muscle stretching (Bayliss effect)
Demonstrating opposite reactions of vessels and intestine on Adrenaline and Acetylcholine
Examining the effects of the cholinergic receptor antagonist Atropine
Comparing the effects of adrenergic α- and β-receptor antagonists (Phentolamine and Propranolol)
Illustrating the effects of the Ca²⁺-channel blocker Verapamil
Recording of dose-response curves of Adrenaline and Acetylcholine
Demonstrating alterations of dose-response curves by competitive and non-competitive inhibitors

for details see Tutorial and Protocol form

download a fully functioning DEMO Version

SimMuscle©

SimMuscle offers a fully equipped, realistically appearing laboratory on the computer screen to perform classical experiments with isolated nerve-muscle preparations of the frog.

All stimulation and recording parameters of the virtual devices are freely adjustable. Mathematical algorithms guarantee for the appropriated reactions of the virtual muscle, also considering the biological diversity of the preparations.

The virtual “SimMuscle” laboratory contains two nerve-muscle preparations and all the apparatus that you will need for experimentation in a simplified but quite realistic form.

When entering the lab you first need to switch on all the devices (POWER buttons). Then drag one of two already prepared nerve-muscle preparations from the Petri-dish to hang it in the suspension apparatus. This includes a mechano-electrical converter transforming changes of either the muscle force or muscle length, selectable by a toggle switch, into an electric potential. You can pre-stretch the muscle hanging one or more weights in the loop at which the muscle is fixed.

Muscle contractions are induced by current pulses delivered from a stimulation apparatus to the electrodes on which the nerve is placed. Stimuli as well as muscle contractions are displayed on a dual beam storage oscilloscope, appropriately displayed with accordingly adjusted voltage amplification and time base (via the rotary switches) and zero lines. Single or double pulses as well as trains of stimuli of selectable amplitude and intervals can be applied.

The example shows muscle contractions, here changes of the muscle length, in response to different trains of voltage pulses inducing isolated twitches, incomplete and complete tetanic contractions depending on the intervals in which the pulses are applied.

Featured experiments:

Single twitches and their stimulus dependencies (recruitement of motor units)
Superposition of single twitches, tetanic contractions, resting tension curves (pre-stretching)
Curves of isometric and isotonic maxima, muscle fatigue

for details see Tutorial and Protocol form

For your own experiments you can download
a fully functioning DEMO Version

SimNerv©

SimNerv offers a fully equipped, realistically appearing laboratory on the computer screen to perform classical experiments of compound action potential recordings from isolated preparations of the frog’s sciatic nerve.

All stimulation and recording parameters of the virtual devices are freely adjustable. Mathematical algorithms guarantee for the appropriated reactions of the virtual nerve, also considering the biological diversity of the preparations.

In the “SimNerv” laboratory you will find a stimulator, an oscilloscope, a recording chamber and a Petri dish with two already prepared nerves.

For your measurements take one of the nerves by mouse click out of the Petri-dish and place it on the electrodes in the recording chamber. The position of the electrodes can be changed and the temperature of the recording chamber can be adjusted. You can set a nerve ligature (fully reversible) using the thread.

The stimulator delivers voltage pulses to the stimulation electrodes. The stimulus is simultaneously displayed at channel 1 of the oscilloscope via a separate cable..AMPLITUDE and DURATION of the stimulus pulses can be adjusted as well as the DELAY between the onsets of two successive stimuli when TWIN pulses shall be applied (MODE). The POLARITY switch allows to invert the direction of the current flow, INVERT corresponds to an exchange of the stimulus electrode positions.

The simple double-beam storage oscilloscope displays the stimulus pulse (channel 1), simultaneously with the compound action potential (CAP, channel 2) measured as the potential difference between the two recording electrodes by means of a differential amplifier. The sensitivity of both oscilloscope channels (mV/DIV) as well as their common time-base (ms/DIV) can be adjusted via the rotary switches.

Featured experiments:

Stimulus-response curve: recruitment of nerve fibres on increasing stimulus amplitudes
Strength-duration curve, rheobase, chronaxy: the impact of stimulus duration
Refractory period and anode break potentials: physiological and clinical relevance of Na⁺-channel inactivation:
Conduction velocity and the effect of temperature changes
Recordings of bi- and mono-phasic action potentials using reversible nerve ligatures
Demonstrating the clinically most relevant impact of appropriate electrode positions
Understanding the difference between intra- and extracellular recordings

for details see Tutorial and Protocol form

For your own experiments you can download
a fully functioning DEMO Version

SimNeuron©

SimNeuron offers virtual laboratories for voltage- and current-clamp experiments in an easy to overlook lab design

towards a better understanding of the relations between ion currents and action potential generation
including a neuron editor showing the full set of the neuron parameters

Featured experiments:

Determine the threshold of action potential generation in thecurrent-clamp lab
Examine the effects of amplitude and duration of the current stimulus
Induction of a series of action potentials of different frequencies
Comparison of action potentials with local potentials and purely passive potential changes
Understanding the effects of the Na-channel blocker TTX and K⁺-channel blocker TEA
Display and explain the alterations of ionic conductances and currents during an action potential

Recordings of ion currents in the voltage-clamp lab with and without RC compensation
Application of the Na⁺-channel blocker TTX and K⁺-channel blocker TEA
Estimation of the activation (and inactivation) time constants
Determining the reversal potentials of Na⁺- and K⁺-currents. (tail currents)
Constructing current-voltage (I-V) curves and calculation of the voltage dependencies of ion channel activation

for details see Tutorial and Protocol form

download a fully functioning DEMO Version

For the experts:

Use the Neuron Editor to examine the effects of different membrane parameters on the neuron’s sensitivity, e.g. with alterations of voltage- and time-dependencies of ion current activation and inactivation, leak conductances, ion concentrations, etc.
Examine how a neuron at constant resting potential can be transferred into a pacemaker neuron.

Pre-Settings:

In fully licensed versions there is the possibility to select to which specific features of the program the students shall have access. This can be done in so-called pre-settings window which you can open from the labs via the SETTINGS button in the switch bank. In demo versions the pre-settings are fixed with most functions enabled.